FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606024
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42570.26506453203688113No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG27840.17334734723765025No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA27270.16979820974032767No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG25030.1558507220315512No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA21920.13648612972159818No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT18110.1127629475026525No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT16370.10192873830029937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7500.031.73980141
TATGCCG7550.029.24393843
GTATGCC8200.027.34653742
CCGTCTT8800.026.26606247
TGCCGTC8100.025.98050945
GGTCCAA11700.025.65288511
GCCGTCT8400.025.46333546
TCGTATG8950.024.67023740
CTCGTAT8650.024.32932739
CGGTCCA12550.023.64054910
TAATCTC9150.023.37690235
ATGCCGT9200.022.87414444
TGCTTGA8350.022.723755
CTAATCT9700.022.40637834
GACGGTC13500.021.9769558
TCGCGTC13200.021.6923730
CTCGCGT13050.021.6773529
GTTCGAA5600.021.56639369
GCTTGAA9600.021.56169356
GTCCAAG13950.021.2680212