FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262851
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG68350.5412356643816254RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG40320.31927757114655647RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG26870.21277252819216202Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG21600.17104155597136955RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG16430.13010244280600006RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13230.10476295303246384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34700.021.97175240
GTATGCC36150.021.5676142
GCCGTCT36450.021.390146
CCGTCTT36950.021.28738447
CGAGGCT3900.021.23665467
ATGCCGT36050.021.05325944
TATGCCG36400.021.04037743
GCTTGAA33300.021.03153256
CGTATGC37050.020.95058641
ACGGGTG7950.020.83596269
GATCTTT23300.020.7287926
GGGGGGG62150.020.65576769
TAATCTC36850.020.59619935
GTCTTCT37450.020.54257849
TGCCGTC38650.020.44032945
CTAATCT37750.020.28793734
CTCGTAT36100.020.1640239
TGCTTGA33200.020.1596455
TCTTCTG38200.020.04894350
CGTGAAA4700.019.8191556
Produced by FastQC (version 0.11.2)