FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595443
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1307908.197723140218734RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG536563.3630784678612775RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG437422.74168365776778Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG304421.908059391654857RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG257261.6124675090241392RNA PCR Primer, Index 29 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG177591.113107770067624RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG100290.6286028394621431Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG76830.4815590403417734RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG67620.4238321268763597RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG62520.3918660835893229RNA PCR Primer, Index 29 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG37120.23266265231662928RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG34190.21429784705564534RNA PCR Primer, Index 29 (96% over 27bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG32120.2013233941920833Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG28800.18051412679738482RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG28260.17712948691993385Illumina PCR Primer Index 12 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG19340.12122025042574382Illumina PCR Primer Index 10 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG17180.10768169091593996Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG17060.10692954872095087RNA PCR Primer, Index 29 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCATG20250.034.75602754
TGCATGA22200.034.34506255
GCATGAA25450.033.34819456
TCTGCAT21900.033.08160453
CATATAC8350.032.236071
TTCTGCA21600.031.4647252
GGGGGGG548550.029.86391469
CATGAAA30800.029.7958657
CGTTAAA2250.029.1337356
TGCGTTA1800.028.75039554
TATTCCG16400.027.55743840
TAGTATG5650.027.47738840
GCCGTCT507950.027.3984746
TGCCGTC516450.027.23477745
ATGCCGT513400.027.21513744
AAGGGGG512250.027.1440969
ATGAAAA38700.027.10114558
TATGCCG517550.027.07690443
GTATGCC527650.026.9443742
CGACACT2050.026.92635223
Produced by FastQC (version 0.11.2)