FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650043
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG2041614.390518539290927TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1343142.8884464079149375TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG1043932.2449899925656602TruSeq Adapter, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG565831.2168274572944808RNA PCR Primer, Index 6 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG285800.6146179723499332RNA PCR Primer, Index 40 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG272190.5853494258010087RNA PCR Primer, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG267460.5751774768534399TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG259360.5577582830954466TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG250730.5391993149310662TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG222650.47881277657002314TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATTCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG222420.47831815748800605TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAGGGGG172870.37176000307954143TruSeq Adapter, Index 2 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG163320.3512225585870926RNA PCR Primer, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG147490.3171798626378294TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG137620.29595425246605245TruSeq Adapter, Index 2 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG137050.29472845734974923RNA PCR Primer, Index 40 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGGATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG111510.23980423406837312TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGG104650.2250516823177764TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCCTCTTCTGCTTGAAAAAAAAAAAGGGGGGG104000.22365384578164116TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG101990.21933130510836135TruSeq Adapter, Index 2 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG92540.19900891239070262TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAAGGGGGGG82610.1776542711540517TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGGGGG81930.17619191908547943TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGGGG73840.15879423050496522TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAGGGGGGG70100.15075129412781774TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG69640.14976205596378356No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG67900.14602015508243688TruSeq Adapter, Index 2 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGG64290.13825678601251642TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATTCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG59980.12898805451906573TruSeq Adapter, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGGCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG58620.1260633503819212TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCCTCTTCTGCTTGAAAAAAAAAAGGGGG58340.1254612054125091TruSeq Adapter, Index 2 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG57570.12380530674662579RNA PCR Primer, Index 40 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG54950.11817095024712675RNA PCR Primer, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG53580.1152247409325032TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGTAAAAAAAAAAGGGGGGG51470.11068714848443337TruSeq Adapter, Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTGGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG51420.11057962259703835TruSeq Adapter, Index 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG50420.10842910484913795TruSeq Adapter, Index 2 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCGCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGG49150.10569794730930446TruSeq Adapter, Index 2 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTTTGCCGTCTTCTGGTTGAAAAAAAAAAGGGGG48430.10414957453081616TruSeq Adapter, Index 2 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG48050.10333237778661403RNA PCR Primer, Index 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAAGGGGGGG46800.10064423060173852TruSeq Adapter, Index 2 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA208.517555E-568.9989335
CATATAC22350.066.544921
TTAATAC3650.064.279892
CTTATAC1281750.064.2204061
ATTATAC6600.060.6497761
ATATACA26300.059.691812
TTATAAC2800.059.1483042
CTTTACA6850.058.9400441
CAGACAC33950.055.3808718
CTAATAC6300.055.321761
CGGAGAT8800.055.2906341
TACCCAT7350.054.9234282
TTTACAC7600.054.47872
CTTAAAC4700.054.331161
ATCACAT2550.054.1226352
ATACCCA7450.053.7300031
GTTTGTA5300.053.3753951
TTTGTAA7000.053.2266152
GACAGAC35600.052.71715516
ACAGACA35750.052.59246417
Produced by FastQC (version 0.11.2)