FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022201
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG124971.2225579900626198Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG72070.7050472460895655Illumina PCR Primer Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG55400.5419677734613838Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG31090.30414761871686685Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG30630.2996475252910142Illumina PCR Primer Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG25040.24496160735510922Illumina PCR Primer Index 10 (96% over 28bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA13920.1361767401910192No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT12120.11856767895942187No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC11130.10888269528204336No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG10520.10291518008689095Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG66900.025.99427469
ACCGGTA4800.025.87443215
GGTCCAA3900.025.65328211
ATATAAG5350.025.1514422
GTCTTAC5050.023.2306211
CATATAA5950.023.196171
AAGGGGG52900.023.02461269
TAGCACT1052.2799853E-523.0006184
ATGCCGT52750.022.62888744
AAAAGGG56600.022.55581967
GACGGTC4750.022.5163968
AAAGGGG55500.022.5055168
AAAAAGG57400.022.481966
TGCCGTC52900.022.36907445
GTATGCC52900.022.30385842
GCCGTCT52500.022.27665146
GTCTTCT52950.022.15248949
CCGTCTT53600.022.14130447
GCTTGAA52200.021.94204756
TATGCCG54150.021.91641643
Produced by FastQC (version 0.11.2)