FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356132
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28420.12062142528517078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT7350.026.28465744
CCGTCTT8550.025.82352347
TCGTATG6200.025.59628740
TGCCGTC8150.025.39774945
GTATGCC7800.025.21052242
GTATCTC8600.022.86584335
GCCGTCT9400.022.754446
AGTCGGA8250.022.58618269
TATCTCG8600.022.46468736
CGTATGC8350.021.48462141
AGTATCT10550.020.92806634
TATGCCG8950.020.81480443
GACGGGT20050.020.30816368
GATCTTT69900.020.1365286
GTCTAGG4300.020.0658471
GAGACTA6900.019.99961937
GTATAAG4050.019.600121
AGACGGG30550.019.42764967
GTCTTCT11550.019.41480349
ACGGGTG20400.019.28313869
Produced by FastQC (version 0.11.2)