FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251315
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT25720.20554376795611015No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA24810.19827141846777188No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC20370.16278874623895662No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC17390.13897379956285988No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC17060.1363365739242317No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA16110.12874456072212034No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG15120.12083288380623584No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG14760.11795591038227785No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA13170.10524927775979669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG5250.024.3143736
GTATCTC5600.024.02686935
GTATGCC4050.023.85098342
CAGTATC8750.021.68492333
TATGCTT11700.021.5257175
AAGGGGG8100.020.87461569
AAAGGGG8600.020.46346368
ATGCTTG12400.020.0323266
GCTTGTC12100.019.9587488
CGTAAAT4850.019.916823
CGCGTAA5250.019.71356621
AGACGGG9150.019.61054267
CGTATGC4600.019.50006741
TATATGC13500.019.4215153
AAGATTA13150.019.4137418
TGCCGTC4800.019.4055445
GTTCGAA4100.019.35759469
GCCGTCT4650.019.2896246
ACGGGTG5400.019.17056569
ATATGCT13550.019.0960064
Produced by FastQC (version 0.11.2)