FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111
Sequences flagged as poor quality0
Sequence length75
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA393.5103510351035103No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG141.2601260126012601RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG100.9000900090009001TruSeq Adapter, Index 5 (96% over 28bp)
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGG50.45004500450045004TruSeq Adapter, Index 5 (96% over 28bp)
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG40.36003600360036003Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG40.36003600360036003RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGG40.36003600360036003RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGGGGGG30.27002700270027RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG30.27002700270027Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG30.27002700270027Illumina PCR Primer Index 10 (96% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGGGGGGGGGGGG30.27002700270027RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG30.27002700270027TruSeq Adapter, Index 12 (96% over 29bp)
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG30.27002700270027Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG30.27002700270027RNA PCR Primer, Index 29 (96% over 30bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG30.27002700270027RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG30.27002700270027Illumina Paired End PCR Primer 2 (96% over 26bp)
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.18001800180018002No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG20.18001800180018002Illumina Paired End PCR Primer 2 (96% over 29bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG20.18001800180018002Illumina PCR Primer Index 10 (96% over 28bp)
AAAGCGCTCCCCAGTACCCCTCGAGGCGCGGCAGGGGGAGGACCCCCCCGGGCCCCGTGGCCGAAAGGATCACGT20.18001800180018002No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGG20.18001800180018002TruSeq Adapter, Index 7 (96% over 28bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGGGGGGGGG20.18001800180018002RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG20.18001800180018002Illumina Paired End PCR Primer 2 (96% over 29bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG20.18001800180018002Illumina PCR Primer Index 7 (96% over 27bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG20.18001800180018002RNA PCR Primer, Index 29 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG20.18001800180018002RNA PCR Primer, Index 29 (96% over 30bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAACAAGGGGGGGGGGGGGGGG20.18001800180018002RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGG20.18001800180018002Illumina Paired End PCR Primer 2 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG20.18001800180018002RNA PCR Primer, Index 29 (96% over 30bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGGGGGG20.18001800180018002RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGGGGGG20.18001800180018002RNA PCR Primer, Index 47 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAA200.005562922551.7521
ACTGCAG200.005562922551.7517
AATCTCG200.005562922551.7526
TTCCGTC200.005562922551.7537
AGCTAAT200.005562922551.7522
GCAGCTA200.005562922551.7520
GCTAATC200.005562922551.7523
TAATCTC200.005562922551.7525
CTAATCT200.005562922551.7524
GACTGCA200.005562922551.7516
TCCGTCT200.005562922551.7538
AGACTGC200.005562922551.7515
CTGCAGC200.005562922551.7518
TGCAGCT200.005562922551.7519
GAGACTG200.005562922551.7514
CGAGACT303.93545E-446.013
CATCTCC358.4268907E-439.4285741
ATCTCCG400.001627665634.52
TGCCGTC500.00487514627.635
GCCGTCT500.00487514627.636
Produced by FastQC (version 0.11.2)