FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071014
Sequences flagged as poor quality0
Sequence length75
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG52019212.777946722855779RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG3526038.661306495138557RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG2206345.41963255346211RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG1629384.002393506875682RNA PCR Primer, Index 41 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG1451583.5656472809968225TruSeq Adapter, Index 3 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG834402.0496122096362233RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG632771.554330198815332Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG488271.1993817756460676RNA PCR Primer, Index 41 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG348860.8569363799780596TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG337360.8286878895528239RNA PCR Primer, Index 41 (96% over 29bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG251950.6188875793598352TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG237970.5845472405646358RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG231260.5680648604991287Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG225960.5550459909988027RNA PCR Primer, Index 41 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG191320.4699566250570496RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG173830.42699435570597394Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG156640.3847690034964262Illumina PCR Primer Index 11 (96% over 26bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG131590.32323642217884785RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG125030.30712250068410474Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG114960.28238664863348545Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG91360.2244158334999585Illumina PCR Primer Index 11 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGGGG90200.22156642055271733Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG84140.20668069429385405Illumina PCR Primer Index 11 (96% over 26bp)
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG81180.1994097784974456RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG79620.1955778093615006RNA PCR Primer, Index 41 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG77730.19093523136987492Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGG77640.19071415622741655RNA PCR Primer, Index 41 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG76860.18879817165944407RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAAGGGGGGG74470.18292739843193856Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG72510.17811287310728974RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG72130.17717944472802108RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCCTCTTCTGCTTGAAAAAAAAAAAGGGGGGG69810.17148061883353877Illumina PCR Primer Index 11 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGG68540.16836100293440406Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAGGGGG63670.15639838133693473Illumina PCR Primer Index 11 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAGGGGGGG59640.1464991277357435Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG59060.1450744212621229Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG57560.14138983555448348TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG55400.13608403213548273Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG52550.1290833192909678RNA PCR Primer, Index 41 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG47880.1176119757878504RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAAGGGGG46650.11459061550758608Illumina PCR Primer Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG45440.11161838303675693Illumina PCR Primer Index 11 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGGG44400.10906373694612694Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG44040.10817943637629347RNA PCR Primer, Index 41 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCCTCTTCTGCTTGAAAAAAAAAAGGGGGGGG41190.10117872353177858Illumina PCR Primer Index 11 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAA150.002355991268.999955
TAGCCTC150.002356220768.9982140
GGGCCCA150.002356335568.9973513
CATATAC22150.065.5909961
CTTATAC1188250.064.9003751
ATATACA24900.062.637962
ATTATAC6750.062.3723221
CTTTACA6500.062.1166951
TTAATAC3600.061.3445472
TTAGGCA401.196895E-860.37269252
CTTAAAC3900.060.170021
TTTACAC7000.058.6607252
CGGAGAT8500.058.056781
TTATAAC1800.057.5105132
GTGTGTT900.057.497847
TCTAGAG309.132229E-657.49779550
TTTGTAA800.056.06035652
TTTATAC1300.055.745751
GTGTATG252.5682925E-455.19856639
CCTGGAT252.5682925E-455.19856636
Produced by FastQC (version 0.11.2)