FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552713
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG12170.22018660679231356Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11800.21349235498350863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTA350.001347385239.4275425
TCGTATG3300.033.4536740
GCTTGAA3050.031.67129956
CCGTCTT3350.030.89471247
GTCTTCT3350.030.89471249
ATGCCGT3350.030.89471244
CGTATGC3600.030.66586141
GATTCGG450.00462122930.66586132
ATGTCAC802.758792E-630.18670854
CTTCTGC4350.030.1371451
TATGCCG3550.030.12596943
TGCTTGA3250.029.72229855
GTATGCC3600.029.70755442
TGCCGTC3600.029.70755445
GCCGTCT3500.029.57065446
GTGCGTT605.419436E-428.74924742
TCTTCTG3750.028.51925350
CGTAGTT500.00773249127.59927724
TTCTGCT4050.027.25854352
CTCGTAT4050.024.70305439
Produced by FastQC (version 0.11.2)