FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543774
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32400.20987527967176542No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG22910.1484025511506218RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC11250.029.74561942
TCGTATG10850.029.25242640
CCGTCTT11950.027.71450647
CGTATGC12100.027.08582541
TATGCCG11900.026.6713343
GCCGTCT12050.026.62561846
CTCGTAT10050.026.08863439
TGCCGTC12600.026.01098845
ATGCCGT12400.025.59587344
GTCTTCT12500.024.56313549
TGCTTGA11550.023.59657355
GCTTGAA12000.022.99919156
TCTTCTG14650.022.37122750
AGACGGG11800.021.6404467
TCTCGTA12600.021.3563938
AAGGGGG16350.021.10564269
CTTGAAA13750.020.82472257
GTCGTAT3000.020.69927239
GACATCT14100.019.81845334
AAAGGGG17150.019.718768
Produced by FastQC (version 0.11.2)