FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168470
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4130.2451475040066481RNA PCR Primer, Index 41 (96% over 29bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT2900.172137472547041No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC2880.17095031756395798No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA2680.15907876773312757No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG2400.142458597969965No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA2380.14127144298688193No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG2270.1347420905799252No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG2250.13355493559684217RNA PCR Primer, Index 41 (96% over 29bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC2240.13296135810530063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.13296135810530063No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA1920.11396687837597198No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA1860.11040541342672286No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC1730.1026889060366831No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG252.560881E-455.21
GTCAACT200.007348193351.75000428
GGACCCA200.007348193351.75000427
GGTAAGC306.296051E-446.029
CGCTGTG306.296051E-446.044
GTGGGAT400.002590075434.548
AGAAACG501.8547002E-434.567
CAAGGGG400.002590075434.53
ATATATA851.198041E-732.470592
TGTTATC1450.030.93103223
TCTTGGG450.00461161530.6666662
GTTTAAT450.00461161530.6666661
ACGGGGG450.00461161530.66666669
GTGCTAT450.00461161530.66666644
GATGAAC450.00461161530.66666652
GTCCAAT450.00461161530.6666661
CCAAACG450.00461161530.66666658
GCATGTT1501.8189894E-1229.920
CAATATA1501.8189894E-1229.941
TATATAC703.8317543E-529.5714283
Produced by FastQC (version 0.11.2)