FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191436
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT7660.40013372615391046No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC7100.37088112998600054No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA7100.37088112998600054No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC6080.3175996155373075No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC5690.2972272717775131No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG5390.2815562381161328RNA PCR Primer, Index 41 (96% over 29bp)
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA5140.26849704339831587No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG5040.26327336551118913No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA4800.2507365385820849No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG4460.23297603376585385No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT4330.22618525251258906No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA3900.20372343759794395No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC3790.1979773919221045No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTT3680.1922313462462651No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC3530.18439582941557492No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG3460.18073925489458617No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGAT3400.17760504816231013No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA3170.16559058902191856No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT3110.1624563822896425No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG3110.1624563822896425No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT3030.15827743997994106No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT2980.1556656010363777No Hit
ATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTACAATTGTG2830.14783008420568755No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCT2790.14574061305083683No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAG2750.14365114189598613No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA2650.13842746400885936No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA2650.13842746400885936No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT2640.13790509622014668No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGG2620.13686036064272133No Hit
TTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCA2590.1352932572765833No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG2580.13477088948787064RNA PCR Primer, Index 41 (96% over 29bp)
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACA2570.13424852169915794No Hit
GTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAA2540.1326814183330199No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG2480.12954721160074384No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTAC2460.12850247602331852No Hit
ATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGT2420.1264130048684678No Hit
ATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATG2410.1258906370797551No Hit
GATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTT2290.11962222361520299No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA2280.11909985582649033No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCT2270.11857748803777765No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG2250.11753275246035229No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATG2210.11544328130550158No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGC2190.11439854572807623No Hit
TCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACA2160.1128314423619382No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCT2160.1128314423619382No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGT2080.10865250005223678No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC2030.1060406611086734No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCC2010.10499592553124804No Hit
AATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAAC1990.10395118995382269No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTC1970.10290645437639734No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1970.10290645437639734RNA PCR Primer, Index 41 (96% over 29bp)
CTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAA1960.10238408658768466No Hit
CCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATC1920.10029461543283394No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAG150.002352611968.9994855
TATAGTG150.002352611968.999485
GAGTGTA200.00734993751.74960726
AACGTAT200.00734993751.74960719
ACTACGG200.00734993751.74960730
TAGGATG200.00734993751.7496074
GAGACGG400.00259105134.49973766
GGTCCAA1850.031.7024611
CGCAGGG450.00460744730.6744462
CTTAGGA450.00460744730.6744462
AGAAGGG500.007719370527.5997920
GTGAGGA500.007719370527.5997919
AAGGCAA500.007719370527.5997943
GACGGTC2150.027.2788628
GGGGGGG4350.026.96531369
CGACGGT2200.026.6588887
TCACATA658.631026E-426.538269
ATCACAT658.631026E-426.538268
TTGTCTC3150.026.28551510
CGGTCCA2100.026.28551510
Produced by FastQC (version 0.11.2)