FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538771
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG718362.8295580814496466RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG322531.2704178517873412RNA PCR Primer, Index 41 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG239760.9443939606998819TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG211890.8346164344873958RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG154130.6071047762874241RNA PCR Primer, Index 41 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG105730.41646135078744795RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG72590.28592574911246427Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG59470.2342472007124707TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG48330.1903677015374762RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG47280.1862318420999767RNA PCR Primer, Index 41 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG31150.12269716331248468RNA PCR Primer, Index 41 (96% over 27bp)
ATTTAATATATTTGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGCCATGC29790.11734024061248534No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTG28970.11411033133748574No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG27210.1071778431374866RNA PCR Primer, Index 41 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA43250.047.233451
CCGATCT46200.042.2654844
TCCGATC48300.042.0706833
TTCCGAT52900.038.6118132
CGATCTG31400.032.6315885
GGGGGGG425750.028.8391569
TCTGCGT21100.027.95867252
TGTCGTA36700.027.6365738
GTCGTAT37200.027.45058439
CGTGAAA26450.026.73865756
CGATCTA9450.026.6503225
TGCGTGA24000.026.16203754
GCGTGAA28200.025.56869555
TTCTGCG21750.025.53697651
GTGAAAA32900.025.3764557
TCTTCCG15400.025.3144875
CTGCGTG22250.025.2732153
ATGTCGT44400.025.25243637
GACATGT48100.025.24645634
GCCGTCT333700.025.05989846
Produced by FastQC (version 0.11.2)