FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205241
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49200111.699709957170112Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG3217237.650524666719457Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1945704.62684540553086Illumina Paired End PCR Primer 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG1579063.7549809868209696TruSeq Adapter, Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1575673.746919617686596TruSeq Adapter, Index 1 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG1268643.01680688455192Illumina Paired End PCR Primer 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG739251.7579254078422615Illumina Paired End PCR Primer 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG522651.2428538578407278RNA PCR Primer, Index 1 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG493181.1727746400265764TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG373420.8879871569786368TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG366650.8718881985598448TruSeq Adapter, Index 1 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG348430.8285613119438339Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG320360.7618112731232288Illumina Paired End PCR Primer 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG226390.5383520231064045RNA PCR Primer, Index 33 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG212350.5049651137711251RNA PCR Primer, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG200150.47595369682736377Illumina Paired End PCR Primer 2 (96% over 29bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG186570.4436606605899638TruSeq Adapter, Index 5 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG179220.42618247087384525RNA PCR Primer, Index 33 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG163750.3893950429951577Illumina Paired End PCR Primer 2 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG163350.3884438489970016RNA PCR Primer, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG143210.34055123118984143Illumina Paired End PCR Primer 2 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG117920.28041199065642136TruSeq Adapter, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG98350.23387482429663367TruSeq Adapter, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG87230.20743163114789379Illumina Paired End PCR Primer 2 (96% over 26bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG84030.1998220791626449Illumina Paired End PCR Primer 2 (96% over 29bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG83670.19896600456430438RNA PCR Primer, Index 33 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGG82470.19611242256983608RNA PCR Primer, Index 33 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG72010.1712386995180538TruSeq Adapter, Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG66680.15856403949262363Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG66080.15713724849538946Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGGGG64140.15252395760433232Illumina Paired End PCR Primer 2 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG63760.151620323306084Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAAGGGGGGG62310.1481722450627681TruSeq Adapter, Index 1 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCCTCTTCTGCTTGAAAAAAAAAAAGGGGGGG61340.14586559961723952TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG60150.1430357974727251TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATTCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG59100.14053891322756532TruSeq Adapter, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG57000.13554514473724574TruSeq Adapter, Index 1 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG56940.1354024656375223Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGG54150.12876788750038345Illumina Paired End PCR Primer 2 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG53730.12776913380231955RNA PCR Primer, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49230.11706820132306328TruSeq Adapter, Index 1 (96% over 26bp)
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG47920.11395304097910203Illumina Paired End PCR Primer 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG46460.11048118288583222RNA PCR Primer, Index 1 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG45740.10876903368915122TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG45370.10788917924085682RNA PCR Primer, Index 33 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAGGGGG44040.10472645919698777TruSeq Adapter, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG43900.10439354129763312Illumina Single End Adapter 1 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG42700.10153995930316478RNA PCR Primer, Index 33 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGT253.0962037E-668.9986837
GGTCTAA150.00235616268.9986836
CATATAC24000.065.705771
ATTATAC5750.064.811861
CTTATAC1286300.063.5428051
TTATAAC1650.062.732042
ATATACA27400.060.946242
TTAATAC3350.060.7658122
AATATAC1600.060.386051
TTAACAC401.1959855E-860.379592
CTTTACA7200.059.90681
GTGTGTT1400.059.14102647
CTAATAC6150.058.9132161
TGTGTTT2350.057.2535448
TTTACAC8000.056.929332
CAAATAC1700.056.8339271
TTTATAC1700.056.8339271
CGGAGAT7750.056.100591
TACCCAT5000.055.2041972
CTTAAAC4650.054.9132771
Produced by FastQC (version 0.11.2)