FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695346
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG28500.16810727721656818Illumina PCR Primer Index 10 (96% over 28bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT24310.14339255821525518No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA23770.14020736769957284No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC18110.10682185229445788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8700.029.34351243
CCGTCTT8650.027.9178247
GCCGTCT9150.027.14632246
TAATCTC8850.026.50806635
ATGCCGT9750.026.1834444
TGCCGTC9800.025.69782445
GTCTTCT9150.025.63819349
CGTATGC10600.024.7354741
AGACGGG12050.024.6300267
GTATGCC10700.024.18115842
AATCTCG9900.023.69660636
GACGGTC7950.023.432918
CTCGTAT10500.023.32821339
GCTTGAA9950.023.23148356
GGTCCAA7650.022.9989711
CGGTCCA7650.022.9989710
ACGGTCC8400.022.9989689
GTCTTAC8400.022.5949341
TGCTTGA10100.022.20328355
ACGGGGG7800.022.12222169
Produced by FastQC (version 0.11.2)