FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132460
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG9450.7134229201268307Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG6270.47335044541748456Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG3990.3012230107202174Illumina Paired End PCR Primer 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG3620.2732900498263627TruSeq Adapter, Index 5 (96% over 28bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT3520.26574060093613167No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3320.25064170315566964TruSeq Adapter, Index 1 (96% over 26bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA2890.2181790729276763No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC2440.18420655292163673No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC2240.16910765514117468No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC2000.15098897780462028No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT1940.14645930847048166No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG1860.14041974935829685Illumina Paired End PCR Primer 2 (96% over 29bp)
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG1780.13438019024611203No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA1780.13438019024611203No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG1720.12985052091197344No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA1610.12154612713271931No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA1560.11777140268760382No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG1490.1124867884644421Illumina Paired End PCR Primer 2 (96% over 29bp)
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG1410.10644722935225728No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC1350.10191756001811869No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTT1340.10116261512909558No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTGA200.00734474451.75000414
ATAACCG200.00734474451.7500045
GAACACT200.00734474451.75000433
CCTGTAC350.001343508439.4285743
GAATAAA400.002588084234.51
GTTAGAG450.00460809230.66666662
GAGCGGG450.00460809230.66666669
AGAGCGG450.00460809230.66666668
GAAACGG953.1677882E-729.05263168
GGTGCTC605.396669E-428.758
AGGGTTC1103.6119673E-828.22727445
AGAAACG1004.9614937E-727.667
CGCTCTT500.007710641727.649
AGTTAGA500.007710641727.661
GGAGGTA1004.9614937E-727.613
GATGTTT756.123071E-527.59999836
AAACGGC1155.5604687E-827.00000269
GGACTCA906.8459303E-626.83333441
AATACAG906.8459303E-626.83333435
GACTCAT906.8459303E-626.83333442
Produced by FastQC (version 0.11.2)