FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015246
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG37600.37035358917937133Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21170.20852089050338538Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG13690.13484416584748918Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10420.10263522338428321TruSeq Adapter, Index 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCT2001.8189894E-1224.15781269
GGGGGGG43600.021.7673669
GTGTAGG2900.021.420721
GATCTTT21950.021.217186
TGTAGGG1657.899507E-820.9127642
GTATGCC22850.020.6834442
ACCGTTA7350.020.6516028
CAGGGGT3350.020.595564
GTTATCG3550.020.4070111
TATGCCG22450.020.28364643
ATGCCGT22600.020.1490244
TTATCGT3600.020.12357712
TCGTATT2750.020.07229837
ATCTTTT21500.020.0567237
GTATACG1551.0044369E-620.03182836
TCGTATG23000.019.94957240
TGCCGTC23000.019.9485945
GGTATAT1751.467306E-719.7206631
GTATCGA1056.0092186E-419.7206631
GGTAGCA1056.015204E-419.7177492
Produced by FastQC (version 0.11.2)