FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572553
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG836245.31772220077797Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG372622.3695226806346117Illumina Paired End PCR Primer 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG292301.858760881191286TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG234261.4896795211353766Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG232471.4782967569296552TruSeq Adapter, Index 1 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG152010.9666446854255469Illumina Paired End PCR Primer 2 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG111090.7064308802310637Illumina Paired End PCR Primer 2 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG70740.44984175414119587TruSeq Adapter, Index 5 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG57500.3656474535357473RNA PCR Primer, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG56670.36036941203253564Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG52860.3361412938069496TruSeq Adapter, Index 1 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG42650.2712150242312978Illumina Paired End PCR Primer 2 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG39360.25029363080290457TruSeq Adapter, Index 1 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG31210.1984670786930552RNA PCR Primer, Index 1 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG29060.18479504347389245Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG27570.17532000511270526Illumina Paired End PCR Primer 2 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG25960.16508187641370434RNA PCR Primer, Index 33 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG19460.12374781644879378RNA PCR Primer, Index 1 (96% over 26bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG17510.1113475984593206TruSeq Adapter, Index 5 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG16710.10626032954056239TruSeq Adapter, Index 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA18100.046.3331381
TTCCGAT19100.044.0809972
TCCGATC22350.037.9737473
CCGATCT21000.037.293444
TGCATGA20850.032.59659655
TCTGCGT23150.031.5924452
TTCTGCA21100.031.06486352
CATATAC7550.030.6261141
CGTGAAA31000.030.60434556
TGCGTGA26400.030.44837254
TTCTGCG23300.029.76037651
GGGGGGG500800.029.75869269
CTGCATG21100.029.59430954
GTGCTCT5900.029.2470441
GCGTGAA30800.029.12290855
TGTCGTA100050.028.99953338
GTCGTAT99150.028.81042939
TCTGCAT22400.028.64594853
TGCTCTT5800.028.556712
TAGTATG5800.028.55126440
Produced by FastQC (version 0.11.2)