FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123133
Sequences flagged as poor quality0
Sequence length75
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG44944814.390933719441342TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG2705178.661718857314114TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG1526614.888072329932795TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1430044.578863596266953TruSeq Adapter, Index 7 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG1258464.029479372156101RNA PCR Primer, Index 7 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG702052.247902987160649RNA PCR Primer, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG328671.0523727295635503TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG311670.9979402093987032TruSeq Adapter, Index 7 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG309970.9924969573822184RNA PCR Primer, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG253880.812901659967731TruSeq Adapter, Index 7 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG170700.5465665407140843TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG167360.5358721514581671Illumina PCR Primer Index 7 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG143520.4595385467093461RNA PCR Primer, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG104810.3355924963810379Illumina PCR Primer Index 7 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG97000.31058555623471684TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG90430.2895489881474788Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG89880.2877879360244985Illumina PCR Primer Index 7 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG88290.2826968944326098RNA PCR Primer, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG77750.24894873193040448Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG65690.21033366174287166TruSeq Adapter, Index 7 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG64210.20559483057557906Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG60580.19397188656390874TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG59550.19067391622450916TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGGGG58550.1874720032736358Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58460.1871838311080572Illumina PCR Primer Index 7 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGG57740.18487845378342838Illumina PCR Primer Index 7 (96% over 26bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG57690.1847183581358847TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG57520.18417403293423623TruSeq Adapter, Index 7 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAGGGGG52280.16739600907165977Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG47050.15065000433859205Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGG44030.14098022722695447Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAAGGGGG43380.1388989838088868Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG42760.1369137977793453TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG42750.13688177864983656Illumina PCR Primer Index 7 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41720.133583808310437Illumina PCR Primer Index 7 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG39860.12762825022181254Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAAGGGGGGG38700.12391403119879942Illumina PCR Primer Index 7 (96% over 26bp)
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG38500.12327364860862473RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG35260.11289945064779502Illumina PCR Primer Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG35120.11245118283467274Illumina PCR Primer Index 7 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG34890.11171474285597187Illumina PCR Primer Index 7 (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGG33650.1077443707968889TruSeq Adapter, Index 7 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAGGGGGG31720.10156467880170329Illumina PCR Primer Index 7 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA150.00235623668.9977147
TTAGCGT253.0962747E-668.9977152
CATATAC18250.062.2073971
CTTATAC1002200.059.2701951
ATATACA20100.059.221092
TTATAAC1700.054.798442
CTTTACA5300.054.6905631
ATTATAC4550.053.087911
TTAATAC2350.052.8552362
CTAATAC4750.052.305561
GTTCATA200.0073607151.749117
CGGAGAT5050.051.2482341
CTTAAAC3400.050.7457961
CTTAATA2450.050.7043721
TTTACAC6000.050.6039922
AGTATCG1300.050.42140624
ATACCCA4050.050.2696651
CAGTATA2000.050.035351
TACCCAT4000.049.1663782
ACCTCGT3000.048.29839737
Produced by FastQC (version 0.11.2)