FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154713
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA3300.21329817145294838No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG2770.17904119240141422TruSeq Adapter, Index 7 (96% over 28bp)
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA2310.14930872001706386No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG2160.1396133485873844No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG2060.13314976763426473No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC1700.10988087620303401No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTT1680.10858816001241008No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA1660.10729544382178614No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAATGCTTATTGTGTTTGAATGT1570.10147822096397846No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC1550.10018550477335453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT150.00235168669.04
GTATTAA200.007347065551.7500041
ATAGAAT200.007347065551.7500043
TAGGGTG200.007347065551.7500045
GACCGTG400.002589424434.57
TAACATG501.8540019E-434.49999649
TGATCCA450.00461046330.6666664
TGAAGCG450.00461046330.6666665
GACGTTT450.00461046330.66666649
GAGACTT450.00461046330.66666637
GCTAAAT450.00461046330.66666643
ACCGGTA802.741328E-630.18750215
AGATAAC703.8297512E-529.57142846
CCGGTAA703.8297512E-529.57142816
CTCGTAT1551.8189894E-1228.93548436
AAACGGC605.400812E-428.7569
GGGACGT605.400812E-428.7516
GGGAGGT854.3956916E-628.41176212
GAGGTAG854.3956916E-628.41176214
AATACGC854.3956916E-628.41176221
Produced by FastQC (version 0.11.2)