Basic Statistics
Measure | Value |
---|---|
Filename | HW32WAFXX_n01_zt2ss54.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 133984 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA | 309 | 0.2306245521853356 | No Hit |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT | 267 | 0.1992775256747074 | No Hit |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC | 242 | 0.18061858132314307 | No Hit |
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC | 240 | 0.1791258657750179 | No Hit |
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC | 225 | 0.1679304991640793 | No Hit |
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA | 192 | 0.14330069262001435 | No Hit |
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG | 188 | 0.14031526152376403 | No Hit |
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG | 183 | 0.13658347265345117 | No Hit |
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA | 182 | 0.13583711487938857 | No Hit |
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA | 174 | 0.12986625268688798 | No Hit |
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT | 154 | 0.1149390972056365 | No Hit |
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC | 147 | 0.10971459278719846 | No Hit |
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG | 135 | 0.10075829949844758 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACAAGAT | 15 | 0.0023510966 | 68.999245 | 3 |
AACGTAT | 20 | 0.007345239 | 51.74944 | 7 |
CGTTCAT | 20 | 0.007345239 | 51.74944 | 66 |
ACACAGT | 35 | 0.0013436347 | 39.428143 | 6 |
GCCGTCT | 70 | 2.1647793E-8 | 39.428143 | 46 |
CTCATCT | 80 | 1.6170816E-9 | 38.81208 | 34 |
GCGATAG | 45 | 9.969008E-5 | 38.332916 | 12 |
CATCTCG | 75 | 3.975947E-8 | 36.799603 | 36 |
GCTTGAA | 85 | 2.928573E-9 | 36.52902 | 56 |
TAATACG | 90 | 5.118636E-9 | 34.499626 | 20 |
ACCGGTA | 90 | 5.118636E-9 | 34.499626 | 15 |
TGCTTGA | 90 | 5.118636E-9 | 34.499626 | 55 |
AAAAGGG | 100 | 3.7471182E-10 | 34.499626 | 67 |
TGCGATA | 50 | 1.8527942E-4 | 34.499626 | 11 |
TCTTTTT | 40 | 0.0025883259 | 34.499626 | 13 |
TAGTGTG | 50 | 1.8527942E-4 | 34.499626 | 16 |
GGCTGTA | 50 | 1.8527942E-4 | 34.499626 | 59 |
TATAAGA | 90 | 5.118636E-9 | 34.499626 | 3 |
TGCCGTC | 80 | 7.015478E-8 | 34.499626 | 45 |
AAAGGGG | 105 | 6.330083E-10 | 32.85679 | 68 |