FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133984
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA3090.2306245521853356No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT2670.1992775256747074No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC2420.18061858132314307No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC2400.1791258657750179No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC2250.1679304991640793No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA1920.14330069262001435No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG1880.14031526152376403No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG1830.13658347265345117No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA1820.13583711487938857No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA1740.12986625268688798No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT1540.1149390972056365No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC1470.10971459278719846No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG1350.10075829949844758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGAT150.002351096668.9992453
AACGTAT200.00734523951.749447
CGTTCAT200.00734523951.7494466
ACACAGT350.001343634739.4281436
GCCGTCT702.1647793E-839.42814346
CTCATCT801.6170816E-938.8120834
GCGATAG459.969008E-538.33291612
CATCTCG753.975947E-836.79960336
GCTTGAA852.928573E-936.5290256
TAATACG905.118636E-934.49962620
ACCGGTA905.118636E-934.49962615
TGCTTGA905.118636E-934.49962655
AAAAGGG1003.7471182E-1034.49962667
TGCGATA501.8527942E-434.49962611
TCTTTTT400.002588325934.49962613
TAGTGTG501.8527942E-434.49962616
GGCTGTA501.8527942E-434.49962659
TATAAGA905.118636E-934.4996263
TGCCGTC807.015478E-834.49962645
AAAGGGG1056.330083E-1032.8567968