FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65381
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA7071.0813539101573852No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA5650.8641654303237943No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.16212661170676496No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA840.12847769229592695No Hit
GAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGA740.11318272892736421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAC208.4562096E-568.9969463
TATAGAT150.002346007268.996937
GAGACGC200.007307294851.78732766
ACAAATG200.007329467751.74770465
TGCTGAA200.007329467751.74770448
TCATAAC200.007329467751.74770455
AAGGGAA551.4333273E-743.94076569
GAAAAGG459.870327E-538.3609866
ACAGAGG400.002569415434.52488766
AAAGAGG501.834522E-434.52488366
CTCATAA400.002579092534.4984754
AAGAGGG851.1715747E-732.4940167
AAAAGGG851.1715747E-732.4940167
AGAGGGG901.9345316E-730.68878668
CCGGGAG450.00459218530.66530414
AGGAAAA450.00459218530.66530463
AAAGGGA605.3445046E-428.77073768
GAAGAGG1004.866033E-727.61990766
GAGAGGA2250.026.08546869
GAAGGGA700.001314893624.66063168