HW32WAFXX_n01_zt2ss4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HW32WAFXX_n01_zt2ss4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2821596
Sequences flagged as poor quality	0
Sequence length	75
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	5142	0.1822372869822611	Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4327	0.15335292508211665	No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT	4074	0.1443863685658755	No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA	3968	0.14062962947211435	No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC	3413	0.12095991063213868	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	1710	31.068802	42
TGCTTGA	1630	30.691547	55
TATGCCG	1765	29.514277	43
TGCCGTC	1780	29.459372	45
CCGTCTT	1765	29.318819	47
GCCGTCT	1820	28.432808	46
CGTATGC	1850	28.345697	41
ATGCCGT	1915	27.022306	44
GCTTGAA	1860	26.896357	56
CTCGTAT	1985	25.375093	39
TAATCTC	2035	25.090689	35
GTCTTCT	2050	24.906216	49
CTAATCT	2100	23.984655	34
CTTGAAA	2105	23.929804	57
CTTCTGC	2320	23.643353	51
CTGCTTG	2160	23.480211	54
TCTTCTG	2365	22.464125	50
AATCTCG	2290	22.447403	36
TTGAAAA	2385	21.9884	58
AAAGGGG	2575	21.977297	68