FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387730
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG71840.30087153907686376TruSeq Adapter, Index 7 (96% over 28bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC38830.1626230771485888No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG36490.1528229741218647TruSeq Adapter, Index 7 (96% over 28bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT32640.13669887298815192No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA29520.12363206895251976No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC27650.11580036268757354No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC26020.10897379519459904No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG25850.10826182189778576TruSeq Adapter, Index 7 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA54500.037.2306061
TTCCGAT58350.034.8894462
TCCGATC59300.034.2076843
CCGATCT59600.033.919734
TCTTACG15300.022.7778552
CTTACGA15000.022.769053
GTCTTAC18250.022.1229111
GGTCCAA15750.022.12288911
TCTTCCG27150.021.9825125
CGGTCCA16650.021.54865310
ACCGGTA10250.021.54056715
GACGGTC16000.021.3459878
GATCTAG5900.021.0499736
CTCGCGT15650.020.72118229
TCGCGTC15600.020.56645230
TATAAGA11650.020.1365013
TATACCG2402.910383E-1120.1241635
TAACGGG16650.020.09826328
ACGGTCC17700.020.0754369
GGGGGGG67050.020.07216369
Produced by FastQC (version 0.11.2)