FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968941
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG293530.9886690237360728RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG226000.7612141837779868RNA PCR Primer, Index 47 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG139690.4705044660705619TruSeq Adapter, Index 9 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG118380.3987280313081331RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG79860.26898479963057537RNA PCR Primer, Index 47 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG66750.22482764056274612RNA PCR Primer, Index 47 (96% over 29bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC43140.14530433578841748No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG37120.12502774558335783RNA PCR Primer, Index 47 (96% over 29bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA32690.11010660029956809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT61150.031.3158842
CTTCCGA61700.031.0994631
CCGATCT65850.029.863184
TCCGATC66500.029.4675273
GGGGGGG253850.024.0071369
ACCGGTA13450.022.31512515
GTCTTAC19900.020.6375051
TCTTACG15900.020.6155382
CTTACGA15850.020.2428043
CTCGTAT161600.019.83246639
TCTCGTA161950.019.5552838
ATCTCGT159400.019.52183337
TATGCCG168150.019.44974143
AGATCTC157450.019.39112335
CGTATGC170200.019.3573641
GCCGTCT168100.019.31186746
GTATGCC170400.019.21316742
CCGGTAA15650.019.17817316
CCGTCTT170200.019.15466747
GCTTGAA167550.019.0049656
Produced by FastQC (version 0.11.2)