FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7836203
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA141820.180980508034312No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA102220.13044582944061045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT44800.025.0268347
GTATGCC47000.024.66277342
GCCGTCT46050.024.49732246
TGCCGTC47250.023.9481845
GACGGGT37950.023.91401768
CGTATGC48150.023.78759841
TATGCCG49300.023.09231843
CTCGTAT48200.022.83244539
ACGGGTG38250.022.10259269
ATGCCGT53350.021.145344
GCTTGAA53200.020.49238856
AAAGGGG65550.020.1620568
AGACGGG52300.019.9916967
AAGGGGG60500.019.79168369
TGCTTGA55250.019.35737655
TCTCGTA56550.019.27805938
CTTCTGC64500.019.20150451
TCGTATG60300.019.05178840
CTGCTTG57350.018.94922654
GAGGGGT25250.018.85933368
Produced by FastQC (version 0.11.2)