FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752236
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG92320.5268696682410361RNA PCR Primer, Index 47 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG62800.35839921106517614RNA PCR Primer, Index 47 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG46430.264975722448346TruSeq Adapter, Index 9 (96% over 28bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA36220.20670731568122103No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG28920.16504626089179766RNA PCR Primer, Index 47 (96% over 29bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA27220.15534437142028815No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT26670.15220552482656446No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA26050.14866718866636686No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC26040.14861011872829916No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG23100.1318315569363944RNA PCR Primer, Index 47 (96% over 29bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC21250.12127361839386931No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC20450.11670802334845305No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG20410.11647974359618227RNA PCR Primer, Index 47 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG58400.027.23716769
CGGTCCA7400.021.91117310
TTGTCTC14850.021.83739910
GTCTCAA15250.021.71705412
GCTTGTC15450.021.6592188
GTCTTAC8950.021.5954781
TATGCTT15700.021.5346745
ATGCTTG16050.021.0650736
CTAGGGG2800.020.9460813
AACGGGT10800.020.76295322
AAGATTA16000.020.69906618
GGGTAAC11050.020.60540625
GTCCTAG11900.020.5925081
GGTAACG11300.020.4548326
ATATGCT18450.020.3817774
AGATTAA16250.020.38061719
GATTAAG16250.020.38061720
ACGGTCC8000.020.2678369
ATGTCTA16350.020.25596833
TGTCTCA16200.020.2305711
Produced by FastQC (version 0.11.2)