Basic Statistics
Measure | Value |
---|---|
Filename | HW32WAFXX_n01_zt2ss40.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1349337 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 4590 | 0.3401670598227129 | Illumina PCR Primer Index 11 (96% over 29bp) |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT | 2499 | 0.18520206590347704 | No Hit |
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA | 2425 | 0.17971789108280586 | No Hit |
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 2076 | 0.15385333686099173 | Illumina PCR Primer Index 11 (96% over 29bp) |
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC | 2050 | 0.15192646462670187 | No Hit |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC | 1844 | 0.13665970769348204 | No Hit |
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC | 1735 | 0.1285816664035745 | No Hit |
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG | 1724 | 0.12776645122752878 | No Hit |
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG | 1718 | 0.1273217884042311 | No Hit |
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA | 1697 | 0.1257654685226893 | No Hit |
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA | 1367 | 0.10130901324131777 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCATGA | 565 | 0.0 | 29.919165 | 55 |
TCTTGAA | 880 | 0.0 | 29.794273 | 53 |
CGTATTC | 1205 | 0.0 | 29.204325 | 38 |
TCCGTCT | 1065 | 0.0 | 28.50591 | 43 |
GTATTCC | 1355 | 0.0 | 28.262966 | 39 |
ATTCCGT | 1010 | 0.0 | 28.010864 | 41 |
TTGCCGT | 1635 | 0.0 | 27.430101 | 44 |
GGGGGGG | 17555 | 0.0 | 27.34266 | 69 |
TCGTATT | 1225 | 0.0 | 27.319305 | 37 |
GCCGTCT | 5370 | 0.0 | 27.174896 | 46 |
TTCTGCA | 750 | 0.0 | 27.138924 | 52 |
TGCCGTC | 5735 | 0.0 | 27.009388 | 45 |
TATTCCG | 1255 | 0.0 | 26.941166 | 40 |
CTTGAAA | 5970 | 0.0 | 25.252764 | 54 |
GGTTGCC | 835 | 0.0 | 25.20259 | 42 |
TCGCGTA | 2140 | 0.0 | 25.150396 | 35 |
ATATAAG | 735 | 0.0 | 24.880249 | 2 |
CCGTCTT | 6260 | 0.0 | 24.744225 | 47 |
CGCGTAT | 2125 | 0.0 | 24.678497 | 36 |
TTCCGTC | 1265 | 0.0 | 24.54448 | 42 |