FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349337
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45900.3401670598227129Illumina PCR Primer Index 11 (96% over 29bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT24990.18520206590347704No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA24250.17971789108280586No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG20760.15385333686099173Illumina PCR Primer Index 11 (96% over 29bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC20500.15192646462670187No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC18440.13665970769348204No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC17350.1285816664035745No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG17240.12776645122752878No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG17180.1273217884042311No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA16970.1257654685226893No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA13670.10130901324131777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATGA5650.029.91916555
TCTTGAA8800.029.79427353
CGTATTC12050.029.20432538
TCCGTCT10650.028.5059143
GTATTCC13550.028.26296639
ATTCCGT10100.028.01086441
TTGCCGT16350.027.43010144
GGGGGGG175550.027.3426669
TCGTATT12250.027.31930537
GCCGTCT53700.027.17489646
TTCTGCA7500.027.13892452
TGCCGTC57350.027.00938845
TATTCCG12550.026.94116640
CTTGAAA59700.025.25276454
GGTTGCC8350.025.2025942
TCGCGTA21400.025.15039635
ATATAAG7350.024.8802492
CCGTCTT62600.024.74422547
CGCGTAT21250.024.67849736
TTCCGTC12650.024.5444842