FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473852
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG47400.32160623997524856Illumina PCR Primer Index 10 (96% over 28bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT27790.1885535318335898No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA25570.17349096110057186No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC23070.15652860667149754No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21160.1435693678876848Illumina PCR Primer Index 10 (96% over 28bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC18600.1261999169523127No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG16780.11385132292794664No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA16530.1121550874850392No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC16250.11025530378898289No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG16090.10916971310552213No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA15980.10842336951064285No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG15370.10428455502994874Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC9700.025.6110691
GGTCCAA8650.024.7276911
TACCGTT1851.8189894E-1124.2426247
CTCGCGT8350.023.96345929
CGGTCCA8800.023.91416210
TCGCGTC8400.023.82081830
GACGGTC9300.023.3703698
GCCGTCT20050.023.05676746
ACGGTCC9300.022.999419
ATGCCGT20350.022.88639344
CCGTCTT20500.022.71893347
TGCCGTC20400.022.66118645
GTATGCC20400.022.49207142
GCTTGAA20400.022.15459856
TGCTTGA20100.021.97033355
GTCGTAA14450.021.96483634
CGTATGC20800.021.89515741
CTCGTAT20800.021.89515739
CGTCGTA14550.021.57676933
GCGTCGT9600.021.56194932
Produced by FastQC (version 0.11.2)