FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834125
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA51490.2807333197028556No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT47270.2577250732638179No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA45990.2507462686567164No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC39320.21438015402439858No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA37660.20532951679956385No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC36240.19758740543856063No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC33500.182648401826484No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG29380.16018537449737613No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA28340.15451509575410619No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA27980.1525523069583589No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG27500.14993525523069584No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA26490.14442854222040483No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT21630.11793089347781639No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC20750.11313296531043414No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG20660.1126422681114973No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT20340.11089756695972193No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGAT20300.11067947931575No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTT20110.1096435630068834No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA19210.10473659101751516No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG18700.10195597355687318No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA18510.10092005724800654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT10650.026.8865846
AGGCTAG1850.026.11094769
CCGTCTT12550.025.01524547
AAGGGGG22750.024.41803669
CGGTCCA10550.024.19838310
GGGGGGG27250.024.18427869
GTATGCC11150.023.82445142
TGCCGTC11450.023.50153445
AGGGGGG15500.023.37350868
CGTATGC10800.023.31943541
GGTCCAA11300.023.20289811
TATGCCG10150.023.1126643
AAAGGGG25300.022.91158168
ACGGTCC11250.022.6927059
AAAAGGG28700.022.36131567
TGCTTGA10400.022.22534655
CGTCTTC14350.022.11785548
CTCGCGT11450.021.69372429
ATGCCGT10500.021.68511444
GACGGTC12100.021.6688218
Produced by FastQC (version 0.11.2)