FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456065
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT27480.18872783838633578No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA25170.172863162015432No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC22050.15143554717680874No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC17980.12348349833283542No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC17560.12059901171994382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16880.11592889053716696No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG15820.10864899575225007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTG8700.025.77958369
CGAGGGT2300.024.00346667
TAGGACT9650.023.9527134
GAGGGTA3350.023.68998768
GGGTAAC8650.023.53114925
CAGGGTT8850.023.0001639
TAACGGG9000.022.9993728
AACGGGT8400.022.9993722
GACGGGT8950.022.746368
TGGTTGC9150.022.62233215
AGAAACG9200.022.50324867
TCAGGGT9200.022.50015638
AGGACTA10300.022.4411335
GGTAACG9450.022.26923226
CATGGTT9300.022.25745613
GAATCAG9200.022.12515435
GGTTGCA9500.021.78887716
GGGAATC9350.021.76945733
TACCATG9400.021.65366210
GAAACGG9750.021.5877368
Produced by FastQC (version 0.11.2)