FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640812
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11610.18117638246474785No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA10700.16697564964451353No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA9530.14871756458992655No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC9380.14637678445472307No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG9240.14419205632853319No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA8580.13389262373363794No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG8540.1332684156975837No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC7090.1106408743906169No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC6880.10736378220133205No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG450.00461172730.6795061
GCCGTCT3650.028.35475346
GTATTCC1850.026.11088439
TGCCGTC4050.025.55428545
GTATGCC3250.025.47565742
CCGTCTT4300.024.87085747
TATGCCG3350.024.71518943
CGAGGGT851.441287E-424.3555367
TCGCGGA851.441287E-424.3555338
AGGTTCG1602.1955202E-923.72127267
TATGCTT4850.023.4730595
CGGTCCA4200.022.99885610
GCTTGTC4600.022.498888
TCCGTCT1854.820322E-1022.37726443
TCGTATT1103.2613254E-521.9568837
ACGGTCC4350.021.4127279
TCCTACA1154.594786E-521.0022322
GTTAGAG1657.893868E-820.91131462
GTCTTCT4800.020.84271249
CCTAAAT1004.328814E-420.7022022
Produced by FastQC (version 0.11.2)