FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909665
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAATAAAATAGGACGTGCGGTTCTATTTTGTTGGTTTTTGGAATCGCCGTAATGATTAATAGGGACAGTCGG13630.1498353789581879No Hit
GCCTTATACGTCGTATAATTGGAATGAGTACAATTTAAATCCCTTAACGAGGAACAATTGGAGGGCAAGTCTGGT12240.13455502849950257No Hit
GATTAATAGGGACAGTCGGGGGCATTAGTACTCACATGCTAGAGGTGAAATTCTTGGATTTTGTGAAGACTAACT12170.13378551444762632No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG10430.11465759372955979Illumina PCR Primer Index 11 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATT5350.031.59742737
CGTATTC6050.030.79270238
GGGGGGG53000.027.86435169
GCCGTCT19250.026.88251946
TTGCCGT6300.026.83273544
TGCCGTC20500.026.42136245
TCGTTTG4250.025.16414540
GTATTCC7150.025.0903539
TCTTCTG23900.024.6835550
GCGTATT2700.024.27723737
GTCTTCT22650.024.21800449
CGTTTGC6200.023.92688641
GATTCGT1301.8469109E-723.88408344
ATTCCGT5350.023.85928241
CTCGTTT5000.023.45947639
CGTCTTC24800.023.37044748
TCCGTCT5350.023.21443643
GTTTGCC6900.022.99948742
TATTGTA1052.2804952E-522.9994872
ATGCCGT17250.022.79949244
Produced by FastQC (version 0.11.2)