FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206497
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG213771.7718237177547895Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG158111.3104881321710706Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG101580.8419415879194064Illumina PCR Primer Index 10 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG74390.6165784084005181Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG60100.49813634016495684Illumina PCR Primer Index 10 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG50440.418069833576047Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG25040.20754299430500034Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13980.11587264618146585No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG12900.10692111128332686Illumina PCR Primer Index 10 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCAA3050.028.27726211
GTAGACT1155.6423232E-826.99863846
ACGGGTG8400.025.472569
CGGTCCA3750.024.83874710
TCCGTCT2001.8189894E-1224.1487843
GACGGTC3300.024.044248
GACGGGT9350.023.99169568
GTCTTAC3750.023.923751
GGGGGGG194900.022.71820469
GTCCAAG3400.022.32240312
ATGTGTA2500.022.0788864
GTCTAGG3000.021.8534261
GCATGAA4900.021.82633456
GCTAGAA2401.8189894E-1221.56230556
GTGTATA2401.8189894E-1221.5614136
ACGGTCC4000.021.5614139
ATATAAG4200.021.358722
CATGAAA4850.021.34001457
TCGTATT1959.331416E-1021.22969837
TACGATA1154.5914465E-521.00677569
Produced by FastQC (version 0.11.2)