FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917523
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG139491.5202888646933101Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG78870.8595969801301984Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG57070.6220007563843086Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35590.3878921836291842No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG34570.37677529609611965Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG32450.35366960828229915Illumina PCR Primer Index 10 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG25780.28097388294353387Illumina PCR Primer Index 10 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.16893309486519684No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG13860.1510588835375244Illumina PCR Primer Index 10 (96% over 29bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.13634535592023306No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.10441155153603779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT59700.026.06209446
CGTATGC60650.025.93827641
CTTCTGC59650.025.91043351
TATCTCG58700.025.8008236
GTATGCC60800.025.760842
TGCCGTC60400.025.7600545
CCGTCTT60150.025.69505147
TCTTCTG61350.025.64232350
TATGCCG60600.025.61810543
GCTTGAA58350.025.5417156
GTCTTCT60000.025.52929549
ATGCCGT60900.025.49190544
GAGCGGA3950.025.33393769
TCTCGTA60700.025.29172138
TGCTTGA57950.025.24175355
CTCGTAT60550.025.24042539
CGTCTTC61250.025.23358748
TCGTATG62550.025.20553840
CTGCTTG58250.025.1709854
TTCTGCT59050.025.00523652
Produced by FastQC (version 0.11.2)