Basic Statistics
Measure | Value |
---|---|
Filename | HW32WAFXX_n01_zt2ss30.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1610575 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT | 2599 | 0.16137093895037488 | No Hit |
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA | 2481 | 0.15404436303804542 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 2128 | 0.13212672492743274 | Illumina PCR Primer Index 10 (96% over 29bp) |
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC | 1932 | 0.1199571581578008 | No Hit |
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC | 1633 | 0.10139235987147448 | No Hit |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC | 1615 | 0.10027474659671236 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 765 | 0.0 | 25.704983 | 43 |
GTATGCC | 760 | 0.0 | 25.420158 | 42 |
CCGTCTT | 765 | 0.0 | 25.254017 | 47 |
GCCGTCT | 780 | 0.0 | 24.768362 | 46 |
ATGCCGT | 785 | 0.0 | 24.171127 | 44 |
TATCTCG | 815 | 0.0 | 24.127987 | 36 |
TGCCGTC | 825 | 0.0 | 23.835527 | 45 |
ACGGGTG | 675 | 0.0 | 23.006336 | 69 |
CGTATGC | 860 | 0.0 | 22.865477 | 41 |
GCTTGAA | 875 | 0.0 | 22.079226 | 56 |
ACCGGTA | 705 | 0.0 | 21.531158 | 15 |
CTCGTAT | 885 | 0.0 | 21.439926 | 39 |
TGCTTGA | 860 | 0.0 | 21.260883 | 55 |
GTGTAGG | 345 | 0.0 | 21.00709 | 1 |
CTTGAAA | 1025 | 0.0 | 20.194414 | 57 |
CGTCTTC | 965 | 0.0 | 20.020023 | 48 |
GGTCCAA | 590 | 0.0 | 19.880657 | 11 |
GACGGTC | 650 | 0.0 | 19.637774 | 8 |
GACGGGT | 810 | 0.0 | 19.597988 | 68 |
CTTCTGC | 1165 | 0.0 | 19.544378 | 51 |