FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610575
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT25990.16137093895037488No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA24810.15404436303804542No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG21280.13212672492743274Illumina PCR Primer Index 10 (96% over 29bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC19320.1199571581578008No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC16330.10139235987147448No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC16150.10027474659671236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7650.025.70498343
GTATGCC7600.025.42015842
CCGTCTT7650.025.25401747
GCCGTCT7800.024.76836246
ATGCCGT7850.024.17112744
TATCTCG8150.024.12798736
TGCCGTC8250.023.83552745
ACGGGTG6750.023.00633669
CGTATGC8600.022.86547741
GCTTGAA8750.022.07922656
ACCGGTA7050.021.53115815
CTCGTAT8850.021.43992639
TGCTTGA8600.021.26088355
GTGTAGG3450.021.007091
CTTGAAA10250.020.19441457
CGTCTTC9650.020.02002348
GGTCCAA5900.019.88065711
GACGGTC6500.019.6377748
GACGGGT8100.019.59798868
CTTCTGC11650.019.54437851