FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822353
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG106480.5842995292350055Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG62830.34477403664383355Illumina PCR Primer Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG46670.2560974739800686Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG28220.15485473999823307Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG26770.1468979939671403Illumina PCR Primer Index 10 (96% over 28bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT25710.14108133824785868No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA25120.13784376572486232No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC22000.12072304323037304No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA21680.11896707169247671No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG20930.11485151340053218Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG63000.026.56313569
ACGGTCC7550.022.3900879
ATACCGT1854.838512E-1022.3783516
CCGTCTT53750.021.11660647
TATGCCG53000.021.0248743
GCCGTCT53400.020.9965946
GTATGCC54750.020.667942
TGCCGTC54200.020.62302645
GTCTTAC9400.020.558811
CGTATGC54750.020.5424441
TCTCGTA52000.020.36824438
TACGACG13050.020.3562985
AAGGGGG51300.020.31270469
CGGTCCA8500.020.29353710
CTCGTAT52550.020.2863739
TAGACTG3250.020.1692075
CGACGGT8050.020.1428347
ATGCCGT54850.020.12704544
GCTTGAA53700.019.97987656
TGCTTGA53750.019.9612955
Produced by FastQC (version 0.11.2)