FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040247
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18810.18082243928605418Illumina PCR Primer Index 10 (96% over 29bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA17780.17092094473716338No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT17250.16582600094016134No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC14400.13842866165439555No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC11910.11449203890998964No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC11410.10968548815810092No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG10990.10564798552651437No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG10980.10555185451147658No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA10730.10314857913553224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG6400.027.49147836
GTATGCC6900.026.49931742
CCGTCTT6950.025.81228447
TGCCGTC7350.025.34628745
GCCGTCT7050.024.95680446
CGTCTTC7200.024.91602348
TATGCCG7400.024.24261943
ATGCCGT7600.024.05859244
TGCTTGA7200.023.47856155
GTCTTCT7200.023.47856149
CGTATGC7800.023.44170641
GACGGTC5950.023.192688
CTCGTAT7600.023.1507239
GGTCCAA6250.022.0794311
GCTTGAA7350.022.06065856
CGTCGTA9400.022.0207133
CGGTCCA6300.021.90419610
CGGTTGT3550.021.3797315
CGCGTCG6650.021.27012831
ACCGGTA5900.021.05030415
Produced by FastQC (version 0.11.2)