FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739853
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39500.5338898402790825Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21560.2914092394029625Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG14960.20220232938164742Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8090.10934604576855132No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG7910.10691313004069727Illumina PCR Primer Index 10 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCC1251.2635974E-724.8392934
GTATTGG1452.0101652E-823.7956431
CTTCTGC18250.023.25139451
CCGTCTT17750.022.9345647
GTATGCC18000.022.61602642
TATGCCG17750.022.35147943
GCCGTCT17950.022.29463246
TGCCGTC18000.022.04104245
TGCTTGA16750.022.03966955
ATGCCGT18050.021.97998644
GATCTTT20100.021.9695246
GTCTTCT18400.021.93687649
GTCGTAA3200.021.56188834
TCGTATG18250.021.55007440
TCTTCTG19400.021.5174350
TATCTCG17600.021.36587136
CGTATGC18250.021.36103841
CTCGTAT17800.021.31961839
CGTCTTC19100.021.3135348
GCTTGAA17000.021.30965856
Produced by FastQC (version 0.11.2)