FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335036
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG135141.012257347367412Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG86160.6453758550331227Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG62650.4692757348865499Illumina PCR Primer Index 10 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG38980.291977145185598Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG33890.2538508324869142Illumina PCR Primer Index 10 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG27330.2047135807573728Illumina PCR Primer Index 10 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22240.16658726805868906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG94550.024.08635369
GATCTTT38000.023.1515546
ACGTTTG4350.022.99937440
AGAGTCG750.00199350522.99937448
GTATGCC64750.022.1646542
ATCTTTT36700.022.0915097
TATGCCG65250.021.99480443
ATGCCGT65950.021.86597344
CCGTCTT64800.021.8281147
TCGTATG65600.021.77227440
TGCCGTC66350.021.63015745
CGTATGC66200.021.62705641
GCCGTCT65300.021.60814346
CTCGTAT65150.021.34017239
CTTCTGC65300.021.13265851
TTCTGCT62750.021.11177652
TATCTCG64000.021.07677336
GTACTAT2301.4551915E-1121.0049381
TGCTTGA62300.020.9873955
GTCTTCT65500.020.96279149
Produced by FastQC (version 0.11.2)