FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894493
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT16830.1881512767567773No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA15200.16992866349988206No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC12770.14276243637457198No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC10660.11917365479662781No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC9800.10955926988808184No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG9610.10743516159433333No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG9460.10575823399400554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGTC7750.025.8184368
ATGCTTG8200.024.401576
TATGCTT8800.023.913925
TTGTCTC8100.023.85110
GTCTCAA8300.023.2762812
AAGATTA8850.022.9991818
AGATTAA8700.022.60264219
TGTCTCA8550.022.59568611
GTCCTAG4600.022.5067461
TCTAGGG2650.022.1362382
TGCTTGT8900.022.0947177
ATGTCTA8350.021.89742333
GTCTAGG2850.021.796011
TAATCTC4200.021.3563835
GAGACTA1304.921323E-621.23001337
ACCGGTA5050.021.17746215
ATATGCT10150.021.073144
AATCTCG4100.021.03583536
TAAGCCA9400.020.5524623
GCATGTC8650.020.34031531
Produced by FastQC (version 0.11.2)