FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918613
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG83720.9113739953603965Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG47690.5191522436542919Illumina PCR Primer Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG36360.3958141241197327Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG20940.22795235861020907Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG20340.22142077240361285Illumina PCR Primer Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG15320.16677316780842422Illumina PCR Primer Index 10 (96% over 28bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA14610.15904412413061866No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT14310.15577833102732055No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC11900.1295431264308256No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC10400.11321416091433498No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC9450.10287248275389091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG40800.028.16066669
ACCGGTA4700.025.69104415
AAGGGGG36100.024.18088369
CCGGTAA5200.023.884216
GCCGTCT37150.023.4948846
GGTCCAA4150.023.27670311
CCGTCTT37550.023.24460447
AAAGGGG37950.023.09302368
TATGCCG37700.023.06060643
GTATGCC37950.022.99960142
TGCCGTC38600.022.79105645
CGGTCCA4100.022.71911810
ACGGTCC4100.022.7191189
ATGCCGT38450.022.70051844
CTCGTAT36200.022.68192939
TCTCGTA36850.022.4690838
CGTATGC38750.022.4357441
AATCTCG36850.022.3754636
AAAAGGG40400.022.20500267
GTCTTCT38700.022.1972949
Produced by FastQC (version 0.11.2)