FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393332
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG23010.5850019830575697TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG11740.29847558805284086TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG10140.257797484059268TruSeq Adapter, Index 12 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6230.15839036742497434No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG5110.12991569462947333RNA PCR Primer, Index 12 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG4250.1080512137329279RNA PCR Primer, Index 12 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACT200.007355614651.74971452
CTAACAG400.002594337834.499814
GTAGACT501.859259E-434.4998146
GTAGTGT400.002594337834.499818
CGACTCA450.004619157430.66649859
AGGTCGC901.9896288E-730.66649868
GGTCGCG1052.3228495E-829.57126469
ATATCGG953.1949094E-729.05247139
CATATCG1254.134563E-927.59984838
CCTTTAG500.007729042327.5998483
GAAGTAC500.007729042327.59984856
TATACTA500.007729042327.59984847
TACGTCT500.007729042327.5998484
TAACACG809.546519E-525.87485740
CACGACG1208.48886E-825.87485557
ATATCGC700.001332331124.6427239
GATCTTT13000.024.4152496
GTAATGT1001.5603337E-524.14986634
ATCTTTT12950.023.9767027
GTGTAGG1301.8342871E-723.8935951
Produced by FastQC (version 0.11.2)