FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779068
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG70010.8986378595963382TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG65010.8344586095180396TruSeq Adapter, Index 11 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG40950.5256280581412662TruSeq Adapter, Index 11 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG26930.34566944092171675RNA PCR Primer, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG21890.28097675684279166TruSeq Adapter, Index 11 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG19170.24606324480019715RNA PCR Primer, Index 11 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12750.16365708769966167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCT854.4284316E-628.40954642
GATTGTG500.007736364427.59784147
ACTAGTG1404.674803E-1027.10676440
ACGGGGG6350.023.3738869
ACGGGTG8050.023.15442569
AGAGCGG7500.023.01149468
GATCTTT19950.022.1336086
GGGGGGG64000.021.7890169
AAACGGG2700.021.7330867
AGACGGG11950.021.6635467
ACCTAGG800.00289452121.5677381
ATCTTTT19400.021.3385377
TGTCCTG2950.021.04920429
TCTAGGG2650.020.833912
GTGTATA1004.3353642E-420.6983816
ATAAGAG1004.3353642E-420.69838110
TCTGTCC8250.020.48930527
GGACGCA1356.8081645E-620.44809764
GACGGGT8300.020.37765168
TACGGGT1701.07807864E-720.3042668
Produced by FastQC (version 0.11.2)