FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3728.24427480916031No Hit
TGCGTCGCCAGATCGAGCTGGTCAGACAGGCAGTCGTCAACTTCAACGACAACGTGCTGGCCAACCTGGCGCTGG10.7633587786259541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTATCGCCAACACCAATCGCACGAAATATA10.7633587786259541No Hit
ACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACGAAACCCCCCACTTCAACAACACAAAAACAGAA10.7633587786259541No Hit
ATCTATTTTTTTTTTTTTTTTTATTTTTTTTTATATTTTTTTTTTTATTTAATTTTTATTTTTTTTTTTTTTTTT10.7633587786259541No Hit
CTTCACCCAGATCACATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACCAAACAACACCCAGCTGCTGT10.7633587786259541No Hit
TTATCGAGATGTCCAAGTAAACCGAATATTCCTCAAAATTCTTGCAGTTTATTAATTTGAAAGATCAAATTACTT10.7633587786259541No Hit
ACGCAGACAGGGAGCCGGCGATAAATGACAACGCTCGGCCAGTGCCACATTGGTGATCCGTGCATCTGCCTGCAA10.7633587786259541No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAGGAAA10.7633587786259541No Hit
CCTCCAACCATGTCCATCTGCTGGTAAAGGACAGAGGCGATACCGGTGTTATTCCATCCGCCATTCAGCTTGTCG10.7633587786259541No Hit
CTCTCGTGGGAGATCGGAAGAGCACACGTCTCCCACGAGAGATCGGAAGAGCCCACGAGACTGCAGCTAATCGCG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAAACGTCTCCAACTCCACAACGAAACAGAACACTAGCTCACCTAAACACCTCAACATAACACCTAGACAACTA10.7633587786259541No Hit
TTGCAATGCTCTATCCCCAGCACGACGGAGTTTAACAAGATTACCCGGACATCTCTGTCAAGGAGGTACTCGCTG10.7633587786259541No Hit
CCGGAGTGGGGTGCAGGGGAAGCCTGCGCCCGGCCTTGGGGCGGCCTATGCCCTCCGTGGGGTCGGACACGGCCT10.7633587786259541No Hit
ATCTACGACGGGTCCTTCTTTTTTTCGCCTTCACGAAATCAAGATGGTGAACGTACAGAAACAACGCCGCACCTT10.7633587786259541No Hit
ATATTTGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGCCATGCACCACCA10.7633587786259541No Hit
CATATAAACATAACCGAGCCAACGAGAGACAAGATAATCTCGATAGAATACAACTGTAAGAAAAAAAAAAAAAGG10.7633587786259541No Hit
ATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATA10.7633587786259541No Hit
GCATTGGGTGTCGACGTCGGCGCTGTGGTCGACATGCAGATGCTCGGCGCACCAGGCGTCCAGCGCCACCGCCCG10.7633587786259541No Hit
GGTCTGTAACTGACGCTGAGGAGAGAAAGCGTTGGGATCGAACAGGATTAGATACCCTGGTAGTCCACGCAGTAA10.7633587786259541No Hit
CTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAAAAAAGAGAGAAAGAA10.7633587786259541No Hit
GATCTGTACTGGGAGACGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAC10.7633587786259541No Hit
GTGCTCTTCCGATCTCCATTGGGAGACGTGTGCACTTCCGATCTCCAATGGTCCACGAGGACAATTCAGAACGAA10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGTAGGGGGTGTTTTAGGGCGTTCGGTGGATGCCTTGGTATGGAAGGCCGAAGAAGACGTGGCAGCCTGCGATA10.7633587786259541No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
GAATAGAACTAACCACAATTGAAAGTAGTACTACCCGTATGAAGCACAAGTACAACAACGAACGAATTAACCGCA10.7633587786259541No Hit
AATCGCAAGAGCCCCACAGTAGATCGGAAGAGCCCACGAGACTACGCTGCATCTCGGAAGCCGGCATAAGGGTGA10.7633587786259541No Hit
AGCACACGTCTCCCCGCCGAGCTCGGACGCGCACTCGTCTCCCCGCCGAGCTCGGCCAGGCGCACGTCACCCCGC10.7633587786259541No Hit
GGGAGACGTGTGCTCTTCCGATCTGCCCCGGGTCTGTCTATTATACACCTAACCGAGCCCACGAGACGGGGATAG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTATCTTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCATATAACAAAAAAAAAAAATGAATCAAA10.7633587786259541No Hit
CTTCCGATCTCGTCAGGGAGACGTGTGCTCTTCCGATCTCTTCACGGTGGCCTGTGCAAAAGCGAAAAAAGCAAG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAAGTGGCATTAAACAGTGTTCCAAGGGATTACTCTTTTTTTTTTTTTGAGACGGAGTCTTGCTCTGTCCCCCAG10.7633587786259541No Hit
AAAGACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCTATCCAAACAGAGAAAAAAATAA10.7633587786259541No Hit
TACATAAATAGAGCCAACTGATCTTTTACAAAGGAGAAAAGGCAAGACGATCGATCAAAAACAGTCTTTTCTACA10.7633587786259541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACGAGATCCACACACCAAACCGAACAAAGAAGGA10.7633587786259541No Hit
CTTCCGATCTGCTAAGGGAGACGTGTGCTCTTCCGATCTCGGGTGGGAGACGTGTGCTCTTCCGATCTCGGGTGG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTTGTATGTAATCAAATCTTATACAATGTTAATCAAACTTATACTATTATATTAAATCAAATACAATATAAATCA10.7633587786259541No Hit
TTATATACCCTCCTCACACAGATACTGCGGCGCGCACACAATCACCTCACATCATCGTGGCATATCCTGGACCCA10.7633587786259541No Hit
AAACAGGGATACTATTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAAGAATAAAAAAA10.7633587786259541No Hit
AGATTAGCTGAAGTGAGTTTTCATTAGACATTTATACATAGCACTACATATGCTGTCTCTTATACACATCTCCGA10.7633587786259541No Hit
GATCGGAAGAGCACACGTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACAGAGGGGAAGAAAAAAAA10.7633587786259541No Hit
CTCGTCAACGCATCGCTCTAACATAGACGGTGAGGTGTAACTGTCTCTTATACACATCTCCGAGCCCACGAACAC10.7633587786259541No Hit
AAATGATGGGGTGGCAGCTCTTGTCTGCTGTGTCTGTCACCGGCGGCGGTCACTATATGGGTGGTTAGGTAGTAA10.7633587786259541No Hit
GAGTTTTACATGTTCTCGTATGAAATTTGCTGGAGGCCTGGACATTTAGGTTTCCCTTACGTTATTCGTAGGGTT10.7633587786259541No Hit
CTTTAATGCAAATGTGGCAGGCCTTCCCCGTAAGGCAGAGGGCTGTCTCTAATACACATCTCCAAACCACGAGAC10.7633587786259541No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGTAACAGGAGAAGCTTG10.7633587786259541No Hit
GATCGGAAGAGCACACGTCTCCCACCAGAGATCGGATGAGCACCCCTCACCCAGACCAGCTCGGAACACCACAGG10.7633587786259541No Hit
CACCTGGGAGACGTGTGCTCTTCCGATCTAAAAGGGTATACGTGTGCTCTACCCATCGAACCGGGGAGACATATC10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGACAACACCGCTCCCTTGAGAACACACGACAACTCACATGACCGACTACGCAAGAAAAAAAAAACAAGGAAA10.7633587786259541No Hit
GGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAA10.7633587786259541No Hit
GGAGACGTGTGCTCTTCCGATCTCCAGTGGGAGACGTGTTATCTTCCGATCTCCCGAGCGCGACGAGAGCGATAA10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTT10.7633587786259541No Hit
CGTCTCCCGGCGAGATCGGAAGAGCACACGTCTCCCGGCGAGATCGGAAGAGCACACGTCTCCCGGCGAGATCGG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTTCAAGGGGAATTCAATTTCGCTGAGTCCATGCTGGAGACAGTGGGAGGTCGTTAAGCCATTCGTGCAGGTTG10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10.7633587786259541TruSeq Adapter, Index 11 (96% over 30bp)
CGTCTCCCTGTCCAGATCGGAAGAGCACACCTCTCCCTCACCACATCCCAACACCACAAGACGGCCTGACCGGAG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGGCTGTCTCTGGAGGCACCGGCGCCACATGTTCAGTCGGAGCACCGGTATCAGGGCAAAGCAAACTAGTAGGA10.7633587786259541No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTGTCATTTTCCCCCCTTTTTTTGTTTATTTTTATAACCAGGACGCGGAAC10.7633587786259541No Hit
GGCTAGAGCAAATATTTATAGGACCATTCTAACACTGCCCTTTAGCACACATAACCGCTCCCAGCCACTGTAACG10.7633587786259541No Hit
CGTTTACAGGTCCTGGGATATCTGTGGTTATTTTTGAGCTATTCTATGCAATTAAACGATGAGGCTTATGGGACA10.7633587786259541No Hit
AACGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATTTCCATCTATATCAAAAAAAACAA10.7633587786259541No Hit
GAAGACCACACCTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
CTCTAAAGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.7633587786259541No Hit
TGTGCTCTTCCGATCTGCTGTGGGAGACGTGTGCTCTTCCGATCTGCTGTCTTAGACGTGTGCTCTTCCCATCAT10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCTTAACGGGGTGTAGTAGTCCATCATCGGACCAGTCAACACTGAGAATAACTTCCACGATCGCCAAA10.7633587786259541No Hit
TGCCAGTAGTTATATGATTGTCTCAAAGATTAAGAGATGCATCTCTACCTCCCCACGAATGAAAAGTGCAACGCC10.7633587786259541No Hit
CAGCAGCGCGAAACCATACCGAAACTAATATGTACTATTACCTACACATTAATTCAGCGCAGTCGACCGCAGGAG10.7633587786259541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAAAAAACAACAGAAAGAAAAAA10.7633587786259541No Hit
CTTCCGATCTTAAAATTTTTTATATTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTATATAAATTTT10.7633587786259541No Hit
CACATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGCGCTACCACACAGCAACCCAAACGATACGGA10.7633587786259541No Hit
CAGCAACCACGAAAGTCGACTTATAGTGAGTTAAGCCATCCCTGACCTGTAAGATTAAAAGATGCGGTGCAAGAT10.7633587786259541No Hit
ATTTTGCTGTCTTCACCTTGCACATGCTGCCCGGAGTTCTGCTGGTGATGGTGCAGACATATATCCAGTTGCGTT10.7633587786259541No Hit
GTATAGCGCTGATCGCCAATCATTTTCGGCGCGGAAACACTCGATGAGTCAGCTATTACGCACTGTTTAAATGGT10.7633587786259541No Hit
GAACTTACAAGACAAGGAATTTCGCTACATAAGGACATTAATCGTTACCGCAGCAGTTTACTGGGGATTCGATCA10.7633587786259541No Hit
AGTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCTGACCCACCCATAGTGAACCAGGACAATTAAAA10.7633587786259541No Hit
CTACCCCACTGCGCCCACCATCAGACCGGCGCAAGACCGCAACCGAGGTGACATATGCCGCTGTACAAGGCCCCC10.7633587786259541No Hit
AGTCCATGCTGGAGACAGTGGGGAGGTCGTTACGCCATTCATGCAGGTCGGAACTTACCCGACAAGGAATTTCGC10.7633587786259541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAA10.7633587786259541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGC10.7633587786259541No Hit
GTCTTTATGTTATCAGTAAGGCTTTTAGTCAACAATAGGCTGTTAGTAAAGTTTTTGGAGAGTCAAAAATTATAC10.7633587786259541No Hit
CGTGTGCTCTTCCGATCTCGGCGGGAGACGTGTGCTCTTCCGATCTCGGCGGGAGCCGAGTGCTCTTCCGATCTC10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAATAATCCCCTACTAAACACAAAAATACCAACAAAATTAATTAAAACATAAAACAAAAATCCATTCGAATAA10.7633587786259541No Hit
CTCCTTGCTTCGTCGACAGCTGCGATGAAATCCTCGACCAGCTGCTCCACAGTTGAGAAGGTGTAGGGCTCTTCC10.7633587786259541No Hit
ACCCAGTTAAGATCGGAAGAGCCCACGTCTCTAATATCCCTCTCGAATAGCCCCATAAGCGGGGAAAAAAAAGAT10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCAGGATCGGTCGATGGTGCGCCCCTCACGAGGCTCCCCCCTACGTTCTCTTTGCCTGCGCGTACGGGGTTGC10.7633587786259541No Hit
GCATGGGGCGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAACTTCTCTGAAGATAATAAAAAAGGCCG10.7633587786259541No Hit
GTTCACCGCACCGGAGCTGGCGACATTGCCTGCCGTGATGTTGGCGTTGAAGGTGGCGCCCGTGCTGGCGAACAT10.7633587786259541No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACAGCAACAAC10.7633587786259541No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACCAACATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10.7633587786259541Illumina PCR Primer Index 11 (96% over 26bp)
ACTTTAATATAAGCTAATGGAGCTGTAATTACCGCGTCTGCAGTCAACAGACTTGCCCTCCAATTGGTCCTGTTT10.7633587786259541No Hit
CCCCAGTGCAGATCGGAAGAGCACACGTCTCCCTGTGTAGATCGGAAGAGACCACGAGCCACTCGCTAAGAGCGT10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGTTGAATAACGTTTGTCTGTCTGACTGTATCTGTCTTTAATTAAAGGTCATCTATTACGCTACATTAGAAAT10.7633587786259541No Hit
GCCGCACACCGTGCGCCTGGTGCGCGCCCACCTGGAAGAAGACGCGGCCAAGTCCCTGCACGATGACTTCAGCGG10.7633587786259541No Hit
GGTATAGAACAAAGCACAATTGAAAAGTGTACTTTCCTTATTCAGCCCTACTTCTCGTACGCACCGTTTAATCGA10.7633587786259541No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers