FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371121
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG14190.3823550809574236TruSeq Adapter, Index 11 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8880.23927506123339828No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG4830.13014623263032812TruSeq Adapter, Index 11 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA200.007356038351.7483813
AATACGA200.007356038351.7483864
GTAGACT400.002594477534.4989236
GTATGCC5300.033.84842
TGCCGTC5300.033.1970845
CCGTCTT5250.032.85611747
TGCTTGA5050.032.79547555
CGTATGC5400.032.58231741
GCTTGAA5200.031.84945156
ATATCGC553.2534086E-431.36265639
ATGCCGT5500.031.36265644
GCCGTCT5450.031.0173846
TCGTATG5700.030.86745640
GGTTAAT450.004616362530.6698381
TATGCCG5650.030.5300243
GTCTTCT5650.030.5300249
CTTCTGC6200.030.0474551
CTTGAAA5600.029.5744957
ATGGAGC703.844888E-529.5705073
TCTTCTG6200.029.49101450
Produced by FastQC (version 0.11.2)