FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563316
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18120.3216667021707177TruSeq Adapter, Index 11 (96% over 30bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA10240.18178074118256893No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG10040.1782303360813469TruSeq Adapter, Index 11 (96% over 30bp)
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC9700.1721946474092694No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT9500.16864424230804734No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC7550.13402779257113237No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC7290.12941226593954372No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG6170.10952999737270024No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG5980.10615711252653928No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA5950.10562455176135598No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA5660.100476464364584No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT5650.1002989441095229No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGG605.4032076E-428.76411
GTTAGGG809.5388146E-525.8807952
CTAGTGA700.001332643424.6439974
GGGGGGG9800.023.94413669
GGGTAGG750.001986097323.0112781
TATGCCG7800.022.55673443
GGGTAAC3450.021.99906725
GTACCGT1103.2619333E-521.9555636
GTATGCC8050.021.85621542
TTAACCG4900.021.83141767
TAACCGC4750.021.79435268
GTTAGAC953.0619773E-421.7904833
CCGTCTT8300.021.6135447
CCGTAAT800.00288938421.5730741
AGACGGG1605.6917997E-821.56732767
AGAAACG2900.021.41858767
GCCGTCT7900.021.39782546
GGTAACG3400.021.3079226
GGAGACT1304.91627E-621.2298736
CGACGGT3450.021.0009757
Produced by FastQC (version 0.11.2)