FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCTCGAGAGATACTAAGAGGACACGTCAAAATTCTGCGCTCGACACTCAAAAAGAACACCAGGCCCGAACA14.3478260869565215No Hit
GGTTTGTGCTGACGGGAATTGTAAGTTGTACTACCCGTATGAAGCCCAGGTTGATCTACGAACGTGTTAACCGCA14.3478260869565215No Hit
TCCCTATAGAGATCGGAAGAGAACAGGTCTCCCTATAGCTGACTCTTATACCCATATCCGAGCCCCCGAGACTCG14.3478260869565215No Hit
GAGCACACGTCTCCCCCGCAAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATA14.3478260869565215No Hit
GGATAGCACCCATCCCTCCTATATCCTCTACACCTCCGGAACGACGGGCAAGCCGAAAGGGGTGGTCCGGGATAC14.3478260869565215No Hit
GTCCGTTATTGTTATCATTCGCCAGCGGCTCGTCCCCCGGCGGCCCCGTCTCCATTGTCACACTTGGTGCTTGTG14.3478260869565215No Hit
GCGATGGAGGGACGGAGTAGGTTATGTCAGCCTGCCGTTGGTTGTCCAGGTGAAAGGAGGTAGGCTTACAGTTTA14.3478260869565215No Hit
ACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGTGATTAAATACTTGTATTTTTTCATAT14.3478260869565215No Hit
CGTGTGCTTCATACGGGTAGTACAACTTACAATTGTTTTTAGTACTATACATTAATGTATGTAAGCGTATAACCG14.3478260869565215No Hit
CGTCTCCCTAGTCAGATCGGAAGAGCACACGTCTCCATTGGCAGATCGGAAGAGCACACGTCTCCCGGGGCAGAT14.3478260869565215Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCCCTGTGCAGATCGGAAGAGCACACGTCATCGAGCGAATACCCCTATCCCAGCCCACGACACGCTCGCGA14.3478260869565215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14.3478260869565215No Hit
CTGGTTGCTCCTGCCAGTAGTTATATGAATGTCTCAAATATTAAGCAATGCTTGTCTAAGAACACACGCATTAAA14.3478260869565215No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGACCGTTTTAACCGCAACACCTTTAATATACGCTATTGGAGCT14.3478260869565215No Hit
AATTTATTTTGAGCATTTTGAATAAGTCATAAATTCCGGCCTGCTGGGTCCTTCCACTCCTTCGGCCCCCATGAC14.3478260869565215No Hit
CTTCAGATCACCCTGCGGAGACGTGTTAACTTACTATCAACCTGTTTAGAATTCTGCTATTCAGATGTCACGAAA14.3478260869565215No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACATATTTCATTATCCCATGCACAGAATATTCAGGCATT14.3478260869565215No Hit
GTGTCACATCGATATATCCAGAATGATGTGATTTCATTATGTATGCTTGATAGAATAAGTGGGAGCTTCGCTTAT14.3478260869565215No Hit
TATAAATAAAAATAACACATTACAGGTTGACTAAATGAGGTGATATAAGTCTTAAACCAAACGAAATAAGAAGCT14.3478260869565215No Hit
CATATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGAATGCCCTCTTCTCCTAGAAAAAAACAAGGGGG14.3478260869565215No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCT14.3478260869565215No Hit
GTCCCTTGGAACAGGGCATCGTAGAGGGTGAAAATCCCGTACTTGCCATGCAAGTACCGTGCTGTGTGATACACG14.3478260869565215No Hit
CGCATCCTTCTGTCTTTTACATACTTTTCAACGAATCTAGTATACCTTTACACTCTAGTCTTACGGGTGTTACAT14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)