FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGACATCGGCTCCTAAAAAATAATCGATATTTCCTTACCCCGAATGAGGTTCAGCTCCTGCTTGCGAAAGTT111.11111111111111No Hit
ATATTTTATTTTTGGAGCTTGAGCTGGAATAGTTGGAACATCTTTAACAATTTTAATTCGAGCTGAATTAGGACA111.11111111111111No Hit
GAAGAGCACACGTCTCCCGGAGGAGAGCGGGAGAGCTCTCGTCTCCTGGTCGCGCTCGGGACAGCACAGGGGGGC111.11111111111111No Hit
GAAGGTGAATTGGAAATAAGCGACAGGCTAAAAAGGATTTTAGACATACACTTCAAGTGGAAGTGGAAGAAGGTT111.11111111111111No Hit
GATTTATGCATGAATTTCGTAAAGTTTCATTCTTAACCGGTACTATTAGTTCATATCATAGAGGATACAATTCAA111.11111111111111No Hit
CCCTGGTCAGATCGGAAGAGCACACGTCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAA111.11111111111111Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGTGTGCGGTGAGCGGGCACAAGTTCCCCCCGCCCCCGCGCATCCAGCACGCTGATATTGTGGACACCCGCTCG111.11111111111111No Hit
AAATCGGAAGCGCACACGTCTCCCATCACAGATCGGACTTTCTCTTATACAAATCACCCACCCCACCAGACTTCA111.11111111111111No Hit
GGCGGAAGTCTAGTAGTCGTTCTCGCTCCGCAGACTCAAAAACTCCAGCAACCATAGGCTGTAGAAAACCGGCTA111.11111111111111No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)